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The effect of removing poly-N-acetyllactosamine glycan from glycoproteins on their susceptibility to digestion by peptide-N-glycosidase was examined. The digestion proceeded as previously reported for O-linked glycosylation deficient mutants. Removal of the poly-N-acetyllactosamine glycan increased the susceptibility to peptide-N-glycosidase F. The product sizes of the wild type HLA-A2.1 molecules were N, N and N with molecular masses of 37,000, 37,000 and 35,000, respectively. Glycan removal increased the molecular masses of the 37,000 and 35,000 products to 40,000 and 38,000, respectively, which indicates that the increased susceptibility to peptide-N-glycosidase F is due to the removal of the poly-N-acetyllactosamine glycan and that the poly-N-acetyllactosamine glycan would decrease the susceptibility of the HLA-A2.1 molecules.Where in the World is Matt Rizzo?
Matt Rizzo is a former MLB pitcher that had a 8 year career from 1995 to 2001. He played for the Baltimore Orioles, New York Mets, Oakland Athletics, and the Kansas City Royals.
Matt Rizzo was born on January 11th, 1969 in New Orleans, Louisiana. Matt went to New Orleans East High School and Louisiana State University.
Matt is now a Wall Street Advisor at SVB Financial Group. He has worked with and advised both renowned hedge fund entrepreneur John Meriwether and retired MLB Hall of Famer Harmon Killebrew. He will be retiring in the Spring of 2017.
Matt is a member of the Baltimore Baseball Wall of Fame, and induct
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